human bc cell lines sw780 (ATCC)
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Human Bc Cell Lines Sw780, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 354 article reviews
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1) Product Images from "C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways"
Article Title: C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways
Journal: Journal of Cancer
doi: 10.7150/jca.56993
Figure Legend Snippet: Silencing of C19orf10 inhibited the migration and invasion of BC cells. (A-B) The mRNA (A) and protein (B) expression levels of C19orf10 were determined by qPCR and western blot assays, respectively. ** P < 0.01, *** P < 0.001. (C-D) Transwell assays were used to assess the migration and invasion of SW780 cells (C) and J82 (D) cells at 48 h after transfection of negative control or C19orf10-specific siRNA oligos. Representative images of cell migration and invasion in the transwell experiments are shown (C-D), and the relative cell motility was quantitated. n = 3 for each group; *** P < 0.001, compared with the control group.
Techniques Used: Migration, Expressing, Western Blot, Transfection, Negative Control, Control
Figure Legend Snippet: Silencing of C19orf10 inhibited the proliferation and colony formation of BC cells. (A-D) SW780 and J82 cells were transfected with negative control or C19orf10-specific siRNA oligos, and cell proliferation and colony formation were assessed at 48 h after transfection. (A-B) The cell viability after silencing C19orf10 in SW780 (A) and J82 (B) cells was measured using the CCK-8 assay. * P < 0.05, ** P < 0.01, *** P < 0.001. (C)The cell proliferation rates of SW780 cells and J82 cells after silencing C19orf10 were assessed using a colony formation assay, and the number of cell colonies was normalized to that of the negative control group. n = 3 for each group; *** P < 0.001, compared with the control group. (D) Detection of cell proliferation by EdU incorporation assay. The relative proliferation rate was quantitated by calculating the percent of EdU-positive cells. * P < 0.05, *** P < 0.001.
Techniques Used: Transfection, Negative Control, CCK-8 Assay, Colony Assay, Control
Figure Legend Snippet: Deficiency of C19orf10 inhibited the malignant growth of subcutaneous cancer xenografts in vivo . (A) The protein levels of C19orf10 in stable SW780 cells with infection of control or C19orf10-shRNA-expressing lentivirus were determined by western blot assays. (B) The infection efficiency of lentivirus in the above cells was observed under a fluorescence microscope. (C) Growth curves show the tumor sizes in nude mice inoculated with the indicated SW780 cell lines (NC, sh-C19orf10#1, and sh-C19orf10#3 cells) during an observation time of four weeks. n=5 per group; * P < 0.05, ** P < 0.01. (D-E) Mice were sacrificed at 4 weeks after tumor cells inoculation. The images of mice at end-point are shown (D), and the excised tumor tissues were images and weighed (E). * P < 0.05, ** P < 0.01. (F) Representative images of immunohistochemical staining (IHC) analysis of PCNA and Ki67 protein levels in tumor tissues. Scale bars, 100 µm.
Techniques Used: In Vivo, Infection, Control, shRNA, Expressing, Western Blot, Fluorescence, Microscopy, Immunohistochemical staining, Staining
Figure Legend Snippet: C19orf10 promotes malignant behaviors and EMT of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathway. (A) Western blotting analysis of PI3K, p-AKT, total AKT, p21, and p27 expressions in control or C19orf10 siRNA-expressing SW780 and J82 cells. (B) Western blotting analysis of PI3K, p-AKT, AKT (pan), p21, and p27 expressions in control or C19orf10-overexpressed UMUC-3 cells. (C) Western blotting analysis of N-cadherin, Vimentin, Snail, Slug, β-catenin, and p-β-catenin expressions in control or C19orf10 siRNA-expressing SW780 and J82 cells. (D) Western blotting analysis of N-cadherin, Vimentin, Snail, Slug, β-catenin, and p-β-catenin in control or C19orf10-overexpressed UMUC-3 cells.
Techniques Used: Western Blot, Control, Expressing